RSL3 (also known as [1S,3R]-RSL3 to indicate the stereochemical configuration) and a number of other reported compounds [11] covalently interact with the nucleophilic active-site selenocyteine of GPX4 and inhibit its enzymatic activity, resulting in loss of its lipid repair function, accumulation of lethal lipid peroxides, and consequent cell death [12]
Dakin CL, Gunn I, Small CJ, Edwards CM, Hay DL, Smith DM, et al
Thereafter, administer subsequent doses once every 7 days (weekly)
Alexa-Fluor 594-conjugated donkey anti-mouse IgG was diluted 1:500 to 4 g/ml (Invitrogen, A21203) as secondary antibody and applied on sections for 1 h at room temperature
With its knobbly, black-and-pink beadwork skin, forked tongue, and neurotoxic venom delivered through grooved teeth rather than hollow fangs, it has inspired fear since the first settlers encountered it in the Gila River basin